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2nd International Conference and Expo on
Vaccines and Vaccination

November 20-21, 2017 | Dubai, UAE

Program Schedule

  • Keynote Speaker

    Time:

    Title

    Title: Protecting your Child and your Community through Vaccination, HCP as Immunization Advocates

    Litty Varghese
    Senior charge nurse, SEHA/Ambulatory Healthcare Services,UAE
    Biography
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    Biography

    Litty Varghese; RN: RM; MSN is working as senior charge nurse, nursing education, AHS/SEHA, UAE. she has 34 years of experience in the field of PHC and ambulatory health care services, UAE and has expertise as JCIA chapter lead and tracer team member, quality improvement coordinator, diabetic clinic project coordinator, community program organizer, nurse educator, newsletter editor and researcher. She holds masters degree in Community nursing, post graduate diploma in nursing administration and obtained Certification in principles of epidemiology and biostatistics from CDC, Atlanta. She taught vaccination in the residency program in faculty of medicine UAEU for 3 years Awards and recognitions: With her continuance in excellent professionalism, she won 1. the First SEHA BEST EMPLOYEE Award 2012(one among top 12) 2. the 3rd Best Abstract Award at The Pan Arab Hypertension Conference in Abu Dhabi Feb. 5-9, 2000, and actively participated in winning the following Awards: 3. ARAB HEALTH AWARD FOR PHC Chronic Disease program; 2009 4. ARAB HEALTH AWARD FOR Nursing HR development; 2010 Her Philosophy is Passionately leading and caring, the best teacher should be the best lifelong student, and we generate value for ourselves by adding value to others



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    Keynote Speaker

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    Title: Necessity of Certified Bio-Containment Facilities, Establishing/Adopting International Standards Constructional Misperception, How to Overcome

    Ravi Kumar Tummalacharla
    Founder & CEO, Cleanrooms Containments Biography
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    Biography

    Mr. Ravi Kumar Tummalachalra is a Graduate Mechanical Engineer, expert in Design& construction of Bio-Containments (BSL-3 Ag, BSL-3, BSL-2 facilities), Cleanrooms, Vivariums (Animal facilities), HVAC systems and energy efficient systems design. In the past worked for many years with various vaccine manufacturing companies in India, having 16 yrs of experience in Design and Construction of Cleanrooms and Bio containments and Animal Research Facilities.Currently Founder and CEO of Cleanrooms Containments since 2011 He is the Member of ABSA (American Biological Safety Association), EBSA (European Biological Safety Association), IVBWG (International Bio-safety Working Group), Society for Biosafety India , ASHRAE (American Society for heating, Refrigerating& Air-conditioning Engineers



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    Sessions:
    Novel vaccines Research and Development & Recombinant Vaccines & Clinical trails & Public Health Issues in Vaccines

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    Title: Development of a recombinant fusion protein vaccine towards Middle Eastern Respiratory Syndrome (MERS)

    Lee Smith
    Founder and Director, Anglo Biopharma Ltd, Uk

    Biography
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    Biography

    Lee Smith
    Founder and Director, Anglo Biopharma Ltd, Uk

    Dr. Lee Smith is a founder and director of Anglo Biopharma Ltd, a recently founded UK based Biotech start up. Anglo Biopharma works in close collaboration with the University of Reading in the United Kingdom on the development of novel biopharmaceuticals with the aim of developing and licencing these novel products through human clinical trial assessment. Lee has over 20 years of experience working in the biopharmaceutical industry globally including companies such as Ipsen Biopharma, Abcam, Takeda Vaccines, GSK, Emergent Biosolutions, Flugen, CFT, Themis, Clearbridge BioMedics amongst others. Lee is also a keen publisher of content and ideas and with organisations to help them understand both the technical and regulatory aspects of their business to reduce technical and regulatory risks and ensure that their programs remain on track. Lee has held a number of senior roles internationally in companies including VP, Development of SingVax, Chief Executive Officer at Singaporean company, Davos Life Science, Development Director for US company Emergent Biosolutions and Analytical Group Head for GlaxoSmithKline, UK. Lee holds Doctorate and Bachelor degrees in biochemistry from the University of Bath and UWE in the United Kingdom



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    Lee Smith
    Founder and Director, Anglo Biopharma Ltd, Uk

    Anglo Biopharma Ltd. is developing a Rapid Epitope & Antigen Discovery Platform (READ) technology in collaboration with the University of Reading, UK. This technology is being used to generate candidates for use in a safe and efficacious vaccine towards Middle Eastern Respiratory Syndrome (MERS). MERS is an emerging infectious disease related to SARS that was described by the director of the World Health Organisation (WHO) as a "a threat to the entire world". The landscape of this disease and the potential for a vaccine will be discussed. Furthermore, the construction and expression of candidate vaccine constructs from the READ platform will be described, highlighting the expression of good yields of MERS fusion proteins as structurally intact and conformationally correct protective antigens. In addition, data will be discussed that has been obtained from serum analysis following candidate protein immunizations during preclinical studies. This isto understand the affinity of serum antibodies against MERS CoVneutralising epitopes known to be important for protection by end-point titer (EPT) ELISA. The utility and validity of using serum to neutralise the infectious ability of a “pseudotyped” MERS virus will also be discussed and their ability todemonstrate useful vaccine efficacy data compared to the classic downstream challenge models

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    Title: Oral Salmonella-Based Vaccine for Type 1 Diabetes

    Mohamed I. Husseiny Elsayed
    Assistant Research Professor, Beckman Research Institute of City of Hope, USA

    Biography
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    Biography

    Mohamed I. Husseiny Elsayed
    Assistant Research Professor, Beckman Research Institute of City of Hope, USA

    He has completed his master of microbiology and immunology from Faculty of Pharmacy, Zagazig University, Egypt. He received his Ph.D from Institute for Clinical Microbiology, Immunology and Hygiene, Friedrich-Alexander University, Germany and postdoctoral studies from LA Biomed at Harbor-UCLA Medical Center, California, and Childrens Hospital Los Angeles at USC California. Currently he is an Assistant Research Professor at Beckman Research institute of City of Hope, California, USA he is working with Salmonella-based vaccine for over 16 years and I published this methodology in reputed journals.



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    Abstract

    Mohamed I. Husseiny Elsayed
    Assistant Research Professor, Beckman Research Institute of City of Hope, USA

    Type 1 diabetes (T1D) is a metabolic disease caused by the autoimmune destruction of pancreatic insulin-producing beta-cells due to loss of tolerance to specific self-antigens (autoantigens). A promising approach to restore immune balance is the oral administration of diabetic autoantigens which diminishes the islet-specific destructive responses and induces regulatory responses. We recently reported the development of an oral vaccine for T1D based on live attenuated Salmonella expressing preproinsulin (PPI) as the autoantigen combined with host cell expression of TGFβ. We showed that oral vaccination with the combined PPI+TGFβ prevented the onset of diabetes in non-obese diabetic (NOD) mice. In this study we extended this approach by evaluating another autoantigen, GAD65, as well as the addition of a complementary treatment, partial T-cell ablation with anti-CD3. Our results showed that GAD65+TGFβ and the combination of PPI with TGFβ+IL10 and a sub-therapeutic dose of anti-CD3 prevented diabetes in the NOD mice and restored normal glucose tolerance. We also found the oral combination therapy of PPI+TGFβ+IL10 combined with anti-CD3 effectively reversed diabetes in the majority of diabetic NOD mice after replacement of arginine with glutamic acid (R22E) and glutamic acid to glycine (E21G) in Insulin B9-23. Initially we have shown that combined vaccine therapy increases the Tregs in splenocytes, and local Tregs in PLN and pancreas of vaccinated NOD mice. Additionally, the combination therapy significantly increased regulatory cytokines (IL10 and IL2) and inhibited the inflammatory IFNγ. Together these results indicated that the vaccine suppressed the autoimmunity and increased regulatory mechanisms leading to a conclusion that a Salmonella-based oral vaccine expressing autoantigens in combination with tolerogenic cytokines is a promising therapy for the prevention and treatment of T1D

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    Title: Development of Novel Vaccines Utilizing a Recombinant Nanoparticle Technology Platform

    Atin Tomar
    President and CEO, CPL Biologicals Pvt ltd,India

    Biography
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    Biography

    Atin Tomar
    President and CEO, CPL Biologicals Pvt ltd,India

    Atin Tomar is President & CEO of CPL Biologicals, a joint venture of Cadila Pharmaceuticals, India and NovavaxInc, USA. Atin brings with him comprehensive experience in the Biotechnology industry, globally. Atin’s experience and expertise spans from Process Development, Technology Transfer and Manufacturing to Business Development, Licensing and Commercialization. Atin completed his B.Tech (Hons) in Biotechnology and Biochemical Engineering from the Indian Institute of Technology (IIT) Kharagpur, India, MS in Biological Engineering from the University of Georgia, Athens, USA and Post-Graduate Programme in Management (MBA) in Finance and Strategy & Leadership from the Indian School of Business (ISB), Hyderabad, India.



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    Abstract

    Atin Tomar
    President and CEO, CPL Biologicals Pvt ltd,India

    A novel technology platform for recombinant vaccines with significant advantages over conventional vaccines:carefully designed genetic constructs enable production of immunogenic protein-based nanoparticles against key antigenic components of pathogens, which enable functional immunity and protection against infection and disease.The platform is based on genetically engineered baculovirus constructs that in Spodopterafrugiperda (Sf9) [Lepidopteran] cells (BV/Sf9) efficiently produce correctly folded, complex antigens as nanoparticles that induce durable, neutralizing and protective immunity (demonstrated in pre-clinical and clinical studies).Our innovation introduces a significant immunologic advantage, embodied by the presentation of antigens as highly immunogenic particles in their native configuration, and sound principles of development, as a scalable, efficient recombinant vaccine production system. Unlike traditional vaccine manufacturing, infectious agents are not required for manufacturing based on this platform. This platform technology is completely recombinant, enabling efficient and safe manufacture of vaccines against highly pathogenic targets. The platform manufacturing process uses no animal source material, there is no requirements for higher BSL facilities and, therefore, no safety threats.This recombinant technology platform is the basis for a portfolio of novel vaccines that represent important advances and address current unmet medical needs. H1N1 (Swine Flu) and Seasonal Influenza vaccines have already been licensed for commercialization in India. Other products currently under development include vaccines against Rabies Virus, Varicella Zoster Virus, Zika Virus, MERS Virus, Ebola Virus etc. The technology also enables Halal certification for the vaccines.H1N1 (Swine Flu) vaccine and Seasonal Influenza vaccines have already been Halal certified

    Time:

    Title: Immune Response Enhanced by DNA priming followed by Proteinic form of vaccine

    Kripa Nidhana Nand
    Research scientist, The Talwar Research Foundation, India

    Biography
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    Biography

    Kripa Nidhana Nand
    Research scientist, The Talwar Research Foundation, India



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    Kripa Nidhana Nand
    Research scientist, The Talwar Research Foundation, India

    Introduction: Immunization against human chorionic gonadotropin (hCG) prevents pregnancy in sexually active women of proven fertility, as shown by previous Phase II efficacy trials. In order to make the vaccine consistent in its linkage to the carrier, we developed a recombinant vaccine linking hCGβ to LTB, B subunit of heat labile enterotoxin of E.coli which isa potent mucosal adjuvant. The hCGβ-LTB vaccine was fairly immunogenic in mice of different genetic strains.Since a vaccine for control of fertility should ideally be effective in every recipient and be potent enough to generate above protective threshold antibody titres to prevent pregnancy, it was decided to investigate if prime-boost approach employing a combination of anti-hCG DNA and protein vaccines, can enhance the immune response. Methodology: hCGβ-LTB protein vaccine was made and purified using yeast Pichia pastoris pPIC9k/GS115 host-vector system.DNA version of the vaccine was prepared by incorporating the gene encodinghCGβ-LTB in eukaryotic plasmid VR1020(DJ).Mycobacterium indicuspranii (MIP)was used as an immuno-modulator.Female inbred Balb C mice received 100 µg of DNA vaccine in saline along with 5x106 cells of MIP/animal/dose route twice fortnightly followed by 2 µg of alum adsorbed hCGβ-LTB along with 5x106 cells of MIP by intramuscular route. Second group of mice was immunized by only protein version of the vaccine along with MIP. Results and Conclusions: Immunization with the DNA form of the recombinant hCGβ-LTB vaccine twice at fortnightly interval followed by the proteinic form of the vaccine induced distinctly higher antibody response than the proteinic vaccine alone.DNA is not only cheaper to make, it is thermostable and does not require cold chain. Hence the employment of DNA for primary immunization is expected to reduce the cost besides the benefitof enhancing antibody response

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    Title: Leishmania Antioxidants: Peroxidoxin 1 as a Promising Target for Vaccine Development

    Nada Sulaiman Daifalla
    Assistant professor, Imam Abdulrahman Bin Faisal University, Saudi Arabia

    Biography
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    Biography

    Nada Sulaiman Daifalla
    Assistant professor, Imam Abdulrahman Bin Faisal University, Saudi Arabia



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    Nada Sulaiman Daifalla
    Assistant professor, Imam Abdulrahman Bin Faisal University, Saudi Arabia

    Leishmania antioxidants, like peroxidoxins, represent potential vaccine candidates against leishmaniasis. We assessed the immunogenicity and protective efficacy of recombinant Leishmaniadonovaniperoxidoxin 1-GST fusion protein (rPxn1-GST) in BALB/c mouse model. Mice were immunized with three s. c injections of rPxn1-GST with or without CPG-ODN in three-week intervals. Four weeks after the last immunization the mice were challenged with Leihsmania major and the level of protection was evaluated as the reduction in lesion size and parasite burden. Recombinant Pxn1-GST protein with CpG demonstrated significant level of protection as compared to rPxn1-GST without adjuvant and all other control groups. The rPxn1-GST/CpG ODN regimen showed marked reduction in the parasite load and footpad swelling of BALB/c mice. Recombinant Pxn1-GST alone and GST with or without adjuvants gave similar results as controls. There was higher level of IgG2a and IFN- in rPxn1-GST/CpG vaccinated mice, as compared to protein alone or adjuvant alone control groups, which indicates that the protection in this group is associated with a biased Th1-type immune response. Despite the enhanced Th1 immune response in mice immunized with GST/CpG, these mice showed progression of the infection similar to other control groups. This result bespeak that the partial protection seen in mice receiving rPxn1-GST/ CpG is antigen dependent

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    Title: Vaccine for Psychiatric Disorders

    Srinivasa K. Rao
    Founder and President, North East Biotech LLC, New York, USA

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    Biography

    Srinivasa K. Rao
    Founder and President, North East Biotech LLC, New York, USA

    Dr. Srinivasa K. Rao is a biotechnology, life sciences, biomedical scientist with experience in teaching, basic research, R&D, training, liaison and business development. Currently he is popularizing, farming and processing quinoa in India with the support of farmers in India and Bolivia. He edited a book ‘Quinoa: Nutritional & Health Benefits’. He launched a brand, “Dr. Quinoa” in the Indian market to make the grain more affordable. Dr. Rao has teaching experience in cell & molecular biology, developmental biology and biotechnology at City University of New York (CUNY). His Ph.D. thesis from the University of Paris, France in 1988 was on the molecular genetics of hemoglobinpathies. His post-doctoral work at Columbia University & the Albert Einstein College of Medicine in New York was on gene expression and regulation. He was a PI at the LIJMC (Albert Einstein College of Medicine) worked in matrix biology and molecular diseases created an enzyme detection kit. Dr. Rao published over 30 peer reviewed scientific papers. He participated in, presented at, organized, co-chaired and chaired several scientific meetings over the last 25 years in France, India and the USA. Dr. Rao led business development for Shantha Biotech in India & the United States. His efforts led to the addition of new vaccines (dengue, rubella, pertussis, human papilloma virus (HPV) to the company’s pipeline. Currently, Dr. Rao is the founder and President of North East Biotech, LLC. In addition to quinoa, the company’s other projects in the area of malnutrition include the development of a growth meter and Poshak, a software for improving nutrition and food security using Neglected and Under Utilized Species (NUS). The company also created value for small oranges in India. Dr. Rao is frequently invited to lecture and motivate students in pharma, life sciences and biotechnology. His vision is to make the next generation part of the biotech revolution to solve problems such as malnutrition. At present working on book – Vaccines in Psychiatry



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    Srinivasa K. Rao
    Founder and President, North East Biotech LLC, New York, USA

    Mental health is a major public health problem. Mental disorders include: depression, bipolar affective disorder, schizophrenia and other psychoses, dementia, intellectual disabilities and developmental disorders including autism. They are generally characterized by a combination of abnormal thoughts, perceptions, emotions, behavior and relationships with others. Globally, an estimated 300 million people are affected by depression alone. Although there are various strategies to treat psychiatric disorders, there is a need for new ones. We examined the advances in immunology to find the suitable immunological approaches for psychiatric disorders. Recent advances in research show that inflammation as one of the triggers for psychiatric disorders. Treating inflammation using immune therapy approach is gaining momentum. Inflammatory markers can act as detectable secondary biological markers for psychiatric disorders. Treating inflammation can help mitigate the psychiatric disorders to the extent that they are causing it. Some studies have shown that there is an increased proinflammatory response in patients diagnosed with depression, indexed by an elevation in C-reactive protein, and acute phase cytokines such as IL-6 and TNF-alpha. Antidepressants have been shown to suppress the inflammatory response, whereas electroconvulsive therapy results in acute elevations in proinflammatory markers. This demonstrates the nuanced nature between inflammation and depression that warrants further investigation. Intravenous Immunoglobulins(IvIg) are well known as a broad-spectrum treatment option for many diseases involving inflammation, with minimal side effects. We present possible use of IvIgsinitially, and proposeother prophylactic and therapeutic vaccines for psychiatric illness

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    Title: Barriers to Adult Immunizations and strategies to improve coverage

    Litty Varghese
    Head of Nursing Education,SEHA/Ambulatory Healthcare Services, UAE

    Biography
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    Biography

    Litty Varghese
    Head of Nursing Education,SEHA/Ambulatory Healthcare Services, UAE

    Litty Varghese RN, RM, MSN is working as Senior Charge Nurse, Nursing Education, AHS/SEHA, UAE. She has 34 years of experience in the field of PHC and Ambulatory Health care Services, UAE and has expertise as JCIA chapter lead and tracer team member, quality improvement coordinator, diabetic clinic project coordinator, community program organizer, nurse educator, newsletter editor and researcher. She holds Master’s Degree in Community Nursing, Post Graduate Diploma in Nursing Administration and obtained certification in Principles of Epidemiology and Biostatistics from CDC, Atlanta. She taught Vaccination in the residency program in Faculty of Medicine UAE for 3 years. Her awards and recognitions with her continuance in excellent professionalism, she won the first seha best employee award 2012(one among top 12), the 3rd best abstract award at the pan arab hypertension conference in Abu Dhabi Feb. 5-9, 2000, and actively participated in winning the following awards: ARAB HEALTH AWARD FOR PHC Chronic Disease program; 2009 ARAB HEALTH AWARD FOR Nursing HR development; 2010. Her Philosophy is Passionately leading and caring, the best teacher should be the best lifelong student, and we generate value for ourselves by adding value to others



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    Litty Varghese
    Head of Nursing Education,SEHA/Ambulatory Healthcare Services, UAE

    Vaccination against childhood communicable diseases through the Expanded Program on Immunization (EPI) is one of the most cost-effective public health interventions available. Most causes of vaccine preventable illness among children are relatively uncommon due to high rates of vaccination and very effective vaccines Adult vaccination is one of the most important tool for reducing morbidity and mortality not just in the elderly but in other members of the society by increasing herd immunity, reducing severe clinical outcome and helping in eradication of the disease. The immunization program overall has been very successful with kids. So is there is a problem? Yes, there is. We’re not doing so well with adults Adults are recommended to receive vaccinations based on their age, underlying medical conditions, lifestyle, prior vaccinations, and other considerations Although provider recommendation is a key predictor of vaccination, more often clients report not receiving vaccine recommendations at healthcare provider visits. Other barriers to vaccinating adults, including the cost of providing vaccination services, inadequate or inconsistent payment for vaccines and vaccine administration, and acute medical care taking precedence over preventive services. Despite these challenges, we introduced a number of strategies which have been demonstrated to substantially improve adult vaccine coverage. Providers are encouraged to incorporate routine assessment of their adult patients' vaccination needs during all clinical encounters to ensure patients receive recommendations for needed vaccines and are either offered needed vaccines or referred for vaccination

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    Title: Conjugation Technology Current Trends and Impact in Vaccine Industry

    B.M.V. Srikanth Babu
    Supervisor - Formulation Research, BravoVax Co. Ltd, China

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    Biography

    B.M.V. Srikanth Babu
    Supervisor - Formulation Research, BravoVax Co. Ltd, China

    Dr. Srikanth, a proficient with fifteen years of industrial experience in downstream processing, conjugation and formulation research of vaccines and currently working as a Research Scientist, Pilot Research department, BravoVax Co. Ltd., Wuhan, China. Did Master’s in Biotechnology, Doctorate degree (PhD) in Human Genetics and MBA (project management) from India’s reputed universities (Perriyar, Osmania and Manipal Universities) and also authored for ten manuscripts in various journals. During last fifteen years involved in process development, technology transfer and manufacturing of various vaccines (HPV, rHBsAg, Pneumococcal, Haemophillus, Typhoid, Cholera Vaccines etc) in Biotech vaccine industries viz. Shantha A SANOFI company, Indian Immunological and Unique Biotechnics Limited



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    B.M.V. Srikanth Babu
    Supervisor - Formulation Research, BravoVax Co. Ltd, China

    Bacterial diseases are the leading cause for children deaths accounting more than 1.5 million per annum, which could be prevented by vaccines. The introduction of conjugate vaccines against bacterial pathogens such as Haemophilus influenzae type b (Hib), Streptococcus pneumonia and Neisseria meningitidis lead to a substantial reduction in mortality rate and incidence of diseases. The impact of conjugate vaccines is considerable and covers vaccination with either monovalent conjugate or in combination with other vaccines. The preparation of conjugate vaccines is an art of science involving preparation of suitable candidate antigen (polysaccharides) which are covalently bound to a carrier protein that ultimately induces long-term immunity by evoking T-cell responses. The preparations of polysaccharides, selection of carrier protein and conjugation techniques are the major imperative factors for the development of conjugate vaccines. The range of conjugation technology is ever expanding using different carriers/ antigens and conjugation methodologies in manufacturing high-quality conjugate vaccines which ultimately provides a significant protection against infectious diseases. The World Health Organization (WHO) has recommended the widespread immunization of Hib, pneumococcal and meningococcal vaccines in epidemic disease areas/countries. Such initiatives along with continuous development of quality conjugate vaccines will significantly contribute in reducing childhood mortality. Knowing the importance of conjugate vaccines improving global health, BravoVax Co. Ltd. (China) is focusing on the development of conjugate vaccines for various diseases as per international regulations at affordable prices

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    Title: Acceptance of seasonal Influenza Vaccination By Healthcare Workers in Al-wazarat Health care Center- PSMMC, Riyadh, Saudi Arabia

    Mostafa abbas Kofi
    professor, PSMMC, Saudi Arabia

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    Biography

    Mostafa abbas Kofi
    professor, PSMMC, Saudi Arabia



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    Mostafa abbas Kofi
    professor, PSMMC, Saudi Arabia

    Background: Seasonal influenza outbreak in healthcare facilities disturbs work fluency and jeopardizes patients’ safety (1). Thus, influenza vaccination of healthcare workers (HCWs) is recommended by the World Health Organization (WHO), United States (US) Center for Disease Control and Prevention (CDC) and the immunization guidelines set by many countries including Saudi Arabia (2). Despite this strong recommendations influenza vaccination rate among HCWs is still suboptimal all over the globe (3). Objectives: To estimate the rate of influenza vaccination uptake by HCWs in WHC. The study is also intended to determine the role of socio-demographic factors, work related factors and health status of HCWs on acceptance of influenza vaccine. Furthermore, it also aim at studying the effect of the HCWs’ beliefs about influenza vaccine and influenza disease on acceptance of influenza vaccine by HCWs. VII Method: A Cross-sectional study was carried out between 27 May and 15 June 2017. A random job-stratified sample of 240 HCWs was selected from HCWs at Al-Wazarat Health Care Center-PSMMC. 25 items self-administered questionnaire was used to collect the data. . SPSS use to data analysis. Results: Out of the 240 randomly selected HCWs, 83% have completed and returned the questionnaires. A statistically significant association existed between most of the socio-demographic factors and vaccination acceptance. However, Age, marital status and income were not significantly association with the vaccination status. A statistically significant association existed between almost all work and heath related factors and acceptance of the flu vaccine. Unexpectedly the only statistically insignificant association was found between chronic diseases and flu vaccination uptake. There is a positive association existed between believe in vaccine preventability, effectiveness and safety while negative with reduced susceptibility to flu disease. Our results showed that, VIII Predictors of vaccination are non-Saudi nationality, Increasing vaccination belief and clinical. Conclusion: The vaccination rate was higher among, males, Muslims, married, graduated and clinical worker participants. The factors affecting awareness about influenza vaccine was gender, nationality, religion, job category and education level. Key words: Seasonal Influenza, Vaccination, Healthcare Workers

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    Title: Molecular Identification of Human Papillomavirus (HPV) in Cervical Smears and Parafin Embedded Tissue Samples from Women with Normal Epithelia, Low and High Grade Cervical Lesions and Cervical Cancer from the Bogota-Colombia

    Patricia Arce
    Secretaria de Salud de Bogota Colombia, South America

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    Biography

    Patricia Arce
    Secretaria de Salud de Bogota Colombia, South America



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    Patricia Arce
    Secretaria de Salud de Bogota Colombia, South America

    Introduction Worldwide cervical canceristhethirdtype of canceraffectingwomen. Severalstudiesshowedthat in cervical cancer HPV prevalenceiscloseto 90%, demonstratingthat HPV infectionisnecessarybutinsufficientfor cervical cancerdevelopment. So, thedevelopment of molecular approachestodetect viral DNA fromoncogenic HPV typesshowedthebenefit of diagnosingthem in high grade lesions (CIN-2 and CIN-3), considered cervical cancerprecursors. Sincethese molecular techniques are sensitive, reliable and consistent, theywereevaluated as tools in secondarypreventionstrategies and also as approachestostudingtheprevalence and incidence of the viral genotypespresent in sexually active women. Objetive Toestablishtheprevalence of HPV infection in paraffinembeddedsamples and cervical smearsobtainedfromwomenwith normal cervical mucosa, high and low grade cervical lesions and cervical cancer. Methods AllwomenbelongtothePublic Net for Cervical CancerPromotion and PreventionProgram, Bogotá and collectedbetween 2015 and 2016. ParaffinembeddedsampleswereprocessedaccordingtoTomassino et al. And alsousingtheQuiagenQIAmp DNA FFPE Tissue kit. Conventional PCR wasusedtoamplify a región of the L1 gene from HPV usingthe MY09/MY011 primers. HPV positive sampleswerethengenotypedusingthe Linear Array kit from Roche. Results A total of 3528 samples, includingbiopsies and cervical smearswereanalyzed. HPV prevalencewas 34.3% (1211/3528). HPV 16 wasthemostprevalentgenotypefound in theanalyzedpopulation, reaching 42.4% of thesamples, followedby HPV 58 (10.6%), HPV 52 (8.9%) and HPV 31 (6.8%). Single infectionsweredetected in 54.8%, (663/1211). Thehighest HPV prevalencewasobserved in thosewomenamong 14 and 25 yearsold (22.5%; n=272). Thehighest HPV positivitywasobserved in thosesamplesclassified as CIN II and CIN III (58.9%; n=274). CIn II/CIN III samplesalsoshowedthehighestproportion of oncogenic HPV types. Conclusions Thisisthefirstretrospectiveanalysis of the HPV genotypes in thesexually active womenpopulationfrom Bogotá, Colombia. Thisinformationcouldcontributetotheimplementation of integratedactions in ordertostrengthenthe Cervical CancerDetection and Control Programfromthe Capital City. Key Words Cervical Cancer, Cervical Intraepitheliallesions, HPV, Molecular detection

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    Title: Preparation and Immunological Characterization of Conjugated Pia- Rsesc As Candidate Vaccine Against Biofilm Forming Staphylococcus Epidermidis

    Mohammad Ahanjan
    Associate Professor, Payambar aazam university complex, Iran

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    Biography

    Mohammad Ahanjan
    Associate Professor, Payambar aazam university complex, Iran

    Dr. Mohammad Ahanjan, born in 1963, Ph.D of Microbiology, associate professor and scientific member of Microbiology and Virology department of medicine faculty of Mazandaran university of medical sciences and member of Infectious Diseases Research Center with focus on nosocomial infection.Manager of Education development office (EDO) of medicine faculty and referee of some scientific journals.Submitted some antibacterial resistant new Genes in NCBI (AHJ.MAZUMS, TOILEE.IR)



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    Mohammad Ahanjan
    Associate Professor, Payambar aazam university complex, Iran

    Staphylococcus epidermidis as an opportunistic pathogen and the leading cause of morbidity and premature mortality in patients with medical .Developing a strategy to raise opsonic antibodies against polysaccharide intracellular adhesion (PIA) could be promising for elimination of colonizing and biofilm forming S.epidermidis. Following the purification of truncated resist protein and PIA, for the first time, PIA were conjugated to recess as a safe carrier to increase the immunogenicity of PIA and we evaluated it efficacy in mice. Construction of the conjugate was analyzed by using Fourier Transform Infrared spectroscopy (FTIR) and Proton Nuclear Magnetic Resonance spectroscopy (H1- NMR) methods. Afterwards, the immune response was evaluated by measuring total IgG, IgG2a, and IgG2b titers. Immunization of mice with the PIA-rSesC conjugate raised the levels of opsonic antibodies, and the vaccinated mice were protected.when challenged intravenously by wild type S. epidermidis strain 1457. Further studies indicated that the conjugated vaccine could eliminate S. epidermidis biofilm formation in in vitro and in vivo assays. This survey confirms the proposal that immunization of mice with PIA-rises conjugate vaccine could be secure and protected against Staphylococcus epidermidis infection

  • Sessions:
    Emerging and Re-Emerging Diseases & Influenza and Respiratory disease vaccines & Pregnancy & infants/Children Vaccines & veterinary vaccines &Immunology / Animal Models & Human Vaccines - Infectious & Non Infectious Diseases

    Time:

    Title: Immunizing Pregnant Women, Benefits and Challenges

    Litty Varghese
    Senior Charge Nurse, Seha Ambulatory Healthcare Services, UAE

    Biography
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    Biography

    Litty Varghese
    Senior Charge Nurse, Seha Ambulatory Healthcare Services, UAE

    Litty Varghese RN, RM, MSN is working as Senior Charge Nurse, Nursing Education, AHS/SEHA, UAE. She has 34 years of experience in the field of PHC and Ambulatory Health care Services, UAE and has expertise as JCIA chapter lead and tracer team member, quality improvement coordinator, diabetic clinic project coordinator, community program organizer, nurse educator, newsletter editor and researcher. She holds Master’s Degree in Community Nursing, Post Graduate Diploma in Nursing Administration and obtained certification in Principles of Epidemiology and Biostatistics from CDC, Atlanta. She taught Vaccination in the residency program in Faculty of Medicine UAE for 3 years. Her awards and recognitions with her continuance in excellent professionalism, she won the first seha best employee award 2012(one among top 12), the 3rd best abstract award at the pan arab hypertension conference in Abu Dhabi Feb. 5-9, 2000, and actively participated in winning the following awards: ARAB HEALTH AWARD FOR PHC Chronic Disease program; 2009 ARAB HEALTH AWARD FOR Nursing HR development; 2010. Her Philosophy is “Passionately leading and caring, the best teacher should be the best lifelong student, and we generate value for ourselves by adding value to others



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    Abstract

    Litty Varghese
    Senior Charge Nurse, Seha Ambulatory Healthcare Services, UAE

    Abstract: Introduction: Vaccine-preventable infectious diseases are responsible for significant maternal, neonatal, and young infant morbidity and mortality. Changes in the immune response in pregnant women – which are thought to occur in order to allow the woman to tolerate the semi-allogeneic foetus – may interfere with the development of the specific immune response to pathogens. These immunological changes may alter the susceptibility of the woman and the foetus to certain infectious diseases and increase the risk of more serious outcomes. This paper discuss the benefits and challenges of immunization of pregnant women. Immunization of pregnant women can protect them directly against vaccine-preventable infections, and in so doing potentially protect the foetus. It can also directly protect the foetus and infant via specific antibodies transferred from the mother during the pregnancy. Pregnant women and their babies are at increased risk for influenza-related complications, including premature labour and preterm birth. Additionally, pertussis outbreaks continue to occur with infants at highest risk of severe illness and death. However, immunization coverage among pregnant women is suboptimal. Clients report concerns for the safety of vaccines and not receiving vaccine recommendations at healthcare provider visits. Department of Health and Human services recommendation includes Assess the immunization status of each patient, Recommend the indicated vaccines to each patient, Administer any necessary vaccines or, if you do not stock the vaccine, refer the patient to a provider or location that can vaccinate the patient, Document the vaccinations that your patient is given, ideally in your state or local immunization registry. Conclusion: Health care providers play a crucial role in helping keep pregnant women and their new born healthy. Assuring our patients are protected by recommended vaccines is key

    Time:

    Title: Assessment of knowledge and attitude about MERS-cov among healthcare workers in wazarat primary care center of psmmc, riyadh, saudi arabia

    Mostafa abbas Kofi
    Professor, PSMMC, Saudi Arabia

    Biography
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    Biography

    Mostafa abbas Kofi
    Professor, PSMMC, Saudi Arabia



    Abstract
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    Abstract

    Mostafa abbas Kofi
    Professor, PSMMC, Saudi Arabia

    Abstract Background Since its discovery in 2012, MERS presents a challenge for HCWs and for epidemiologists. Its spread in Arabian Peninsula, North Africa, East Asia and Europe represents a burden both Health care economic and human resources Therefore, good knowledge, positive attitude, and healthful practice of healthcare workers (HCWs) regarding MERS-CoV are a cornerstone in prevention of virus spread and disease outbreak. Objectives: The objective of this study is to determine the knowledge and attitude of MERS- COV among healthcare worker in primary care center of PSMMC, Riyadh, Saudi Arabia. Methods: A cross sectional study was performed in primary care center of PSMMC, Riyadh, Saudi Arabia. A total of 233 healthcare workers were selected to participate in this study. Knowledge and attitude were assessed by using self-administered and pretested questionnaire. Descriptive statistics were carried out to express participants’ demographic information, mean knowledge score and mean attitude score of HCWs. Result: Participants demonstrated good knowledge and positive attitude towards MERS. Majority of the respondents had gained knowledge about MERS from internet as shown by this study. The most number of correct responses were gathered from the question about the symptoms of MERS followed by the question on developing severe acute respiratory illness. The lack of respondents’ knowledge was shown in the question asking about the incubation time of MERS-CoV, about 82.4% of the HCWs answered it incorrectly. The most positive attitude of HCWs was regarding the use of protective equipment when dealing with MERS patient (4.64±0.64). Overall, gender and experience were the two demographic variables significantly associated with the mean knowledge and attitude scores Conclusions: The findings of this study showed that healthcare workers in Wazarat primary care center of PSMMC, Riyadh, Saudi Arabia, have good knowledge and positive attitude towards MERS. Yet there are areas where low knowledge and negative attitude of HCWs was observed. However, studies are required to assess the knowledge and attitude of HCWs at national level so that effective interventions could be designed as surveillance and infection control measures are critical to global public health. Key words: Knowledge, attitude, MERS-CoV, healthcare workers

    Time:

    Title: Molecular Identification of Human Papillomavirus (HPV) in Cervical Smears and Parafin Embedded Tissue Samples from Women with Normal Epithelia, Low and High Grade Cervical Lesions and Cervical Cancer from the Bogota-Colombia

    Patricia Arce
    Secretaria de Salud de Bogota Colombia, South America

    Biography
    χ

    Biography

    Patricia Arce
    Secretaria de Salud de Bogota Colombia, South America



    Abstract
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    Abstract

    Patricia Arce
    Secretaria de Salud de Bogota Colombia, South America

    Introduction World wide cervical Cancer is the third type of cancer affecting women. Several studies showed that in cervical cancer hpv prevalence iscloseto 90%, demonstrating that hpv infection is necessary but insufficientfor cervical cancer development. So, the development of molecular approaches to detect viral dna from oncogenic hpv types showed the benefit of diagnosingthem in high grade lesions (cin-2 and cin-3), considered cervical cancer precursors. Since these molecular techniques are sensitive, reliable and consistent, they were evaluated as tools in secondary prevention strategies and also as approaches to studing the prevalence and incidence of the viral genotypes present in sexually active women. Objetive To establish the prevalence of HPV infection in paraffin embedded samples and cervical smears obtained from women with normal cervical mucosa, high and low grade cervical lesions and cervical cancer. Methods All women belong to the public net for cervical cancer promotion and prevention program, bogotá and collected between 2015 and 2016.paraffin embedded samples were processed according totomassino et al. and also using the QIAamp DNA FFPE Tissue Kit. conventional PCR was used to amplify a región of the l1 gene from HPV using the my09/my011 primers. HPV positive samples were then genotyped using the linear array kit from roche. Results A total of 3528 samples, includingbiopsies and cervical smearswereanalyzed. HPV prevalence was 34.3% (1211/3528). HPV 16 was the most prevalent genotype found in the analyzed population, reaching 42.4% of the samples, followed by HPV 58 (10.6%), HPV 52 (8.9%) and HPV 31 (6.8%). Single infections were detected in 54.8%, (663/1211). Thehighest HPV prevalence was observed in those women among 14 and 25 years old (22.5%; n=272). The highest HPV positivity was observed in those samples classified as CIN II and CIN III (58.9%; n=274). CIn II/CIN III samples also showed the highest proportion of oncogenic HPV types. Conclusions This is the first retrospective analysis of the HPV genotypes in the sexually active women population from Bogotá, Colombia. This information could contribute to the implementation of integrated actions in order to strengthen the Cervical Cancer Detection and Control Program from the Capital City. Key Words Cervical Cancer, Cervical Intra epitelial lesions, HPV, Molecular detection

    Time:

    Title: Sub-Domain Frap Of Cell Surface Molecules to Monitor Cell Surface Topography In Living Cells

    Maha Al-Jumaa
    Research Student, Cardiff University School of Medicine, UK

    Biography
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    Biography

    Maha Al-Jumaa
    Research Student, Cardiff University School of Medicine, UK



    Abstract
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    Abstract

    Maha Al-Jumaa
    Research Student, Cardiff University School of Medicine, UK

    It is becoming increasingly clear that many cells have a reservoir of excess plasma membrane in the form of cell surface wrinkles and micro-ridges. The released of these surface structures allow cells to change shape rapidly by permitting an apparent increase in cell surface area eg during phagocytosis or cell spreading. Although indirect methods point to this conclusion, it is only possible to visualise the cell surface topography under scanning electron microscopy and therefore cannot be used to follow surface topography changes to be monitored in living cells during changes in cell morphology. In this abstract, we describe a novel methodological approach which allows this using the measurement of fluorescence recovery after photo bleaching in subdomain at defined 2 dimension distances from the bleach front. Assuming that the diffusion of the fluorescent molecules is constant to rate of recovery at defined points reflects the diffusion distance and hence indicates the surface topography between the bleach front and the measurement domain. Human neutrophils were isolated from blood and there plasma membrane labelled with DiI. The dye can be excited using a 543nm laser line and effectively photobleached using the 488nm line transiently elevated. A photo-bleaching zone across the region of the cell of interest was chosen and the image of the cell recording during bleaching and recovery. The bleach/recovery cycle could be repeated. This approach was showed to reflect the cell surface topography by experimentally increasing the wrinkledness of the cell surface using hyperosmotic media. The measured apparent diffusion length increased in the increased wrinkled state and was returned to close to its pre-shrunk value by restoration of isotonicity. The methodology showed that whereas the body of neutrophils has significant ridge-like topographical features, the phagosome and the extending pseudopodia are smooth. This method also showed that elevation of cytosolic Ca2+ (by uncaging IP3) influenced the cell surface topography. This methodology opens a novel way of monitoring an important cell characteristic which has not be possible to study previously.

    Time:

    Title: Development and Evaluation of Eimeria Tenella(Indigenous/ Local Strain) Vaccine(S) against Coccidiosis In Chickens

    Mian Abdul Hafeez
    Associate Professor, University of Veterinary & Animal Sciences, Pakistan

    Biography
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    Biography

    Mian Abdul Hafeez
    Associate Professor, University of Veterinary & Animal Sciences, Pakistan



    Abstract
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    Abstract

    Mian Abdul Hafeez
    Associate Professor, University of Veterinary & Animal Sciences, Pakistan

    Sporozoites of E.tenella(local isolate) were adapted on the chorioallantoicmembrane(CAM) of chicken embryos. Gametocytes were harvested from CAM and chorioallantoic fluid. Egg adapted gametocytes (E. tenella) were used to prepare adjuvanted(Amphigen) vaccine and compared with the non-adjuvanted by immunizing chicks through oral and subcutaneous route in comparison with controls.Egg adapted gametocytes (EAG)of E. tenella and wall fragments from sporulated and un-sporulated oocysts were subjected to SDS-PAGE to fractionate the proteins. Five random samples taken from different aliquots of EAG were analyzed. All the samples of gametocyte showed similar protein bands having molecular weights of 48.43, 27.20, 25.75, 22.57 and 12.92 kDa. Sporozoites-specific ELISA with purified sporozoites of E. tenella revealed that antibodies produced due to EAG vaccine are sporozoite specific. Humoral response in vaccinated and control chicks was detected by IHA test and ELISA. Results of all groups with IHA geomean titer and ELISA OD values were partially comparable. A significant difference (P>0.01) in geomean titer and OD values among vaccinated and control groups at day 5th and 14th post vaccination was recorded. Cell mediated immunity (CMI) of the vaccinated and control groups were detected by Modified splenic cell migration inhibition assay. There was significant difference (P>0.01) in migration index of vaccinated groups with control group either with or without antigen and non-significant difference (P>0.01) was observed in vaccinated groups with or without adjuvanted vaccine. On 10th days post challenge, organ body weight ratio of Lymphoid was calculated. The ratio of the vaccinated chicks remains normal while increased in non-vaccinated chicks (control groups).Sporulated oocysts of mixed species of genus Eimeria were used to challenge the vaccinated and control chicks. Both vaccines check the mortality but maximum protection (71.42 %) against heavy doses of infection was recorded by adjuvanted vaccine given orally. Maximum reduction (86%) in oocyst count was also observed in group A given adjuvanted vaccine orally.

    Time:

    Title: Development of Goat Warble fly vaccine for the control of P. silenusbraur in local long hairy Goat (Capra hirsa)

    Muhammad Mazhar Ayaz
    Assistant Professor, Bahauddin Zakariya University, Pakistan

    Biography
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    Biography

    Muhammad Mazhar Ayaz
    Assistant Professor, Bahauddin Zakariya University, Pakistan



    Abstract
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    Abstract

    Muhammad Mazhar Ayaz
    Assistant Professor, Bahauddin Zakariya University, Pakistan

    Introduction: Warble fly disease or Grub disease is equally affecting the cattle, buffalo and goats at Pakistan especially in Southern Punjab. The area mostly under attack is near rivers belt of River Sind, River Chennab area and Suleyman mountainous area. The disease has economic effect on the growing economy of the livestock based country/ region. The disease is endemic for centuries in the subcontinent and area reported. Materials and Methods: For the affective control of P. silenusbraurthe somatic vaccine was developed first time in Pakistan. The somatic antigen of the 2nd stage larva was purified by ultracentrifugation at the speed of 3000 rpm for 10 minutes. The supernatant was obtained and desiccated. The 1 µgm of the dried somatic material was mixed with saponins of Sapindustrifoliatus (A new candidate for adjuvant) and injected subcutaneously at 0.5 ml to the suspected Capra hirsaharboring migratory larva of P.silenusbraur. Result: The injected animals showed specific immunity along with increase in Neutrophils. The animals under experiment could not show the nodules or eruptions of L3 in skin of the animals. The study proved that somatic vaccine against P.silenusbraur showed 85 to 95 controls in grub eruption that could be a good candidate for the effective control of P.silenusbraurL3 along with Sapindustrifoliatusa new candidate in adjuvants family. Discussion: Warble fly inflicts economic losses in livestock and tannery industry. It is imperative to control the problem and to address the issues timely. The fly is endemic in area and has been reported from adjacent countries including Iran but little data from Afghanistan about the disease. The ivermectin remained a drug of choice for decades to control the disease but emergence of resistance is a constraint. To address the dipteran disease which completes its partial life cycle inside the host can be justified by the vaccine. This is first time that warble fly in long hairy goats of the area has been treated effectively by the vaccine.

    Time:

    Title: The trans-boundary disease and the problem of continues vaccination

    Samia Ahmed Kamal
    Associate professor, Animal Health Research Institute, Egypt

    Biography
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    Biography

    Samia Ahmed Kamal
    Associate professor, Animal Health Research Institute, Egypt

    Dr. Samia Ahmed Kamal is (Associate professor) first researcher in virology department in AHRI of Egypt. She graduated from Faculty of Veterinary medicine, ZagazigUniversity in 1989. She worked for Animal Health Research Institute in 1991 till now. Her postgraduate studies are; Master degree in Veterinary Pathology, Zagazig University (1996), Ph.D. in Veterinary Pathology, Cairo University (2005) and Diploma of public health (microbiology) from Alexandria University (2009). In the present time, she focused on her researches in the field of virology, vaccines and public health (microbiology)



    Abstract
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    Abstract

    Samia Ahmed Kamal
    Associate professor, Animal Health Research Institute, Egypt

    How to address the continued presence of rift valley fever virus in neighboring countries that do not take any measures to resist the virus and import live animals. This problem is ongoing and cannot be tolerated or ignored. Because the risk of weather fluctuations exists and the possibility of a suitable environment for those viruses exists. The effect of these conditions has been evident in the foot-and-mouth disease, which has always taken place in the Egyptian environment. The various infectious diseases, which do not exist in Egypt, enter through the port of Sudan to represent a direct outlet for other African countries that do not have a border. The continued importation of camels and cattle from Sudan is considered a permanent threat. The continued vaccination of animals with Rift Valley Fever vaccine for decades is also inappropriate behavior to address this problem. As the possibility of cheating in the vaccine, especially in a country like Egypt, puts the country at risk of a pandemic through the vaccine, which has already happened before. The application of vaccine safety tests is not enough, because it does not include the experiment on the target animals so fully that we observe the pathological effect of the tested vaccine, which include strict and rigorous pathological examination. Ignoring the source of natural infection and continuing to import these animals is the real problem that must be faced. The control of disease-carrying insects must be effective and severe. Both in countries exporting disease and receiving countries

    Time:

    Title: Fluorescent Isothiocyanate Dextran Evaluates the Permeability of Blood-Brain Barrier in Rabies Infected Mice Model

    Waqas Ahmad
    Lecturer, College of Veterinary and Animal Sciences, Pakistan

    Biography
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    Biography

    Waqas Ahmad
    Lecturer, College of Veterinary and Animal Sciences, Pakistan

    Mr. Waqas Ahmad, Completed matriculation in 2006 and F.Sc Pre-Medical in 2008, both from Pakistan Urdu School & Higher Secondary Education - Bahrain via F.B.I.S.E - Islamabad. Completed D.V.M with a Gold Medal in 2013 form University of Veterinary Sciences-Lahore. Joined Small Animal Practice as Teaching Assistant at Pet Centre-UVAS after graduation in 2013. Got promoted to the status of Lecturer at Pet Centre in 2015. His hobbies include collecting coins and postal stamps, gardening, video gaming, fish keeping, etc. Area of interest include soft tissue surgery, ultrasonography, exotic animal medicine, human-pet bonding, mobility and pain management, injury and surgery rehabilitation



    Abstract
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    Abstract

    Waqas Ahmad
    Lecturer, College of Veterinary and Animal Sciences, Pakistan

    Rabies is primarily a horrifying viral zoonosis that annually accounts for 55,000 deaths worldwide. Acute encephalitis develops as the rabies virus (RABV) enters to the central nervous system by crossing the blood brain barrier (BBB) which is a tight junction of endothelial cells. In this study, three different molecular weights (70 kDa, 150 kDa and 200 kDa) of fluorescent isothiocyanate dextrans (FITC-Dextrans) were used to measure the extent of BBB damage and subsequent leakage patterns in brain tissues of rabies infected mice which were post-immunized with neutralizing antibodies to observe whether it has positive effect on infected mice by decreasing the death ratio. The brains were processed for immunofluorescence to observe the neutralizing antibodies and its relevant compatibility with the leakage of FITC- Dextrans. Results showed that 70 kDa and 150 kDa FITC-Dextrans efficiently crossed BBB, and produced fluorescent illumination mainly in the cerebral cortex of brain. The enhancement of BBB permeability was significant at 5th day of post-immunization, while the neutralizing antibody neutralized some rabies virus particles by crossing BBB, but it did not present enough treatment effect to the dying mice. These findings suggest that FITC-Dextran is an important fluorescent marker to investigate the integrity of BBB permeability. Keywords: Rabies virus, blood-brain barrier, Evans blue, FITC-Dextrans, neutralizing antibody.

    Sessions:
    Poster Session

    Time:

    Title: Prophylactic of metastases of uveal melanoma by xenovaccination with mouse tumor cells.

    Vladimir Sologub
    Research Center of Molecular Diagnostics, Russia

    Biography
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    Biography

    Vladimir Sologub
    Research Center of Molecular Diagnostics, Russia



    Abstract
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    Abstract

    Vladimir Sologub
    Research Center of Molecular Diagnostics, Russia

    Prophylactic of infectious diseases by live vaccines were the most successful events in the struggle against viruses and bacteria in the XX century. Small pox, poliomyelitis and tuberculosis were taken under control by wide application of corresponding live vaccines. We suggested that live vaccine for oncology could be live xenogeneic tumor. Mouse models have shown that xenotransplantation of tumors are safe for recipients with normal immunity. Moreover, unsuccessful xenotransplantation is very successful immunization. Xenotransplantation of tumors, widely used in experimental oncology, is successful only in immunodeficient mouse models but can be used as powerful immunization tools for creation of anti- tumor immunity in normal subjects. We have shown significant protection for mouse melanoma B-16, when we vaccinated animals with human Sk-Mel -1 melanoma cells transplanted into connective tissue capsule. Xenogeneic transplantation of mouse melanoma cells to humans healthy volunteers – authors of this presentation, have shown raise of serum antibodies against common human/ mouse melanoma antigens, s-100 and GM-3, by ELISA . T-cells anti-melanoma response was detected by gamma-interferon Elispot test. Local immunity, immediate and delayed types, against mouse B-16 melanoma was present in the course of this xenogenic anti-tumor vaccination. Classical vaccination according to Sir Edward Jenner means prophylactics but in oncology, there is a great will to apply vaccines for therapeutic purposes. In case of melanoma, one point of application for prophylactic vaccine is post surgical immunization to prevent future metastasis. It is especially important for uveal melanoma; where primary tumor is removing with the completely affected organ – eye and metastasis usually appear in lungs and liver several years after surgery

    Time:

    Title: Input of newly described Titer Max Gold adjuvant in antiscopionic vaccine approach: Safety and Efficiency evaluation

    Fatima Laraba-Djebari
    Professor, USTHB, Algeria

    Biography
    χ

    Biography

    Fatima Laraba-Djebari
    Professor, USTHB, Algeria



    Abstract
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    Abstract

    Fatima Laraba-Djebari
    Professor, USTHB, Algeria

    Scorpion envenomation is a major public health problem encountered in tropical and subtropical countries such as North Africa, Central and South America, the Middle East, and South Asia. The severity of this accidental pathogenesis is due to the massive release of sympathetic and parasympathetic neurotransmitters, which leads to hemodynamic changes associated to cardiogenic shock and pulmonary edema leading to the death. Right now, the only available specific treatment is the serotherapy which remains limited due, not only to its low efficiency, but also to the delay of its administration. Vaccine could be a promising alternative to this therapy. This approach must be developed in compliance with bioethical rules requiring the use of the highly immunogenic yet biocompatible preparations. In this study, the immunogenic effect of TiterMax Gold (TMG) adjuvant associated to irradiated Androctonus australis hector venom was compared to commonly used Freund’s complete adjuvant preparation. The obtained results showed that this formulation using TMG in the immunization of animals results in low activation of granulocyte cells as well as their related peroxidase activities while the immunized animals with FCA adjuvant present signs of chronic inflammatory response. The specific immune response triggered by this vaccine showed that attenuated venom associated to TMG are highly efficient resulting in high specific titers of antibodies, mainly those of IgG1 subclass, suggesting a potent activation of the humoral immune response by this adjuvant. The evaluation of immunoprotective effect against the native venom showed that TMG preparation was able to protect animals up to 5 LD50 of native venom while FCA preparation was limited to 3 LD50. Moreover, the histological analysis of pulmonary and cardiac tissues of immunized animals with the TMG formulation and survived to the high doses of native venom showed no alterations compared to those immunized with FCA, providing more evidence to the potent immunoprotection conferred by TMG preparation. Altogether, these results give an insight on the harmlessness and the potency of this newly described adjuvant in the induction of a long lasting specific immunoprotection against scorpion envenoming compared to a classic adjuvant. Keywords: Scorpion envenomation, TiterMax Gold, Vaccination, immunoprotection

    Time:

    Title: Strengthening technical assistance for routine immunization training - the START approach in Uganda

    Nicholas Ayebazibwe
    African Field Epidemiology Network, Uganda

    Biography
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    Biography

    Nicholas Ayebazibwe
    African Field Epidemiology Network, Uganda



    Abstract
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    Abstract

    Nicholas Ayebazibwe
    African Field Epidemiology Network, Uganda

    Building health workforce capacity is a key strategy to achieve the Global Vaccine Action Plan’s goal of strengthening routine immunization systems to boost plateauing vaccination coverage. The Strengthening Technical Assistance for Routine Immunization Training (START) approach was designed to utilize practical training methods to develop skills of both district and health facility staff to implement key planning and monitoring activities for routine immunization as well as build supportive supervision skills of district staff. First implemented in Uganda, the approach was executed by trained external consultants who mentored district-level staff and with them, conducted on-the-job training for health facility staff. The START approach was designed to be flexible and adaptable to meet district needs, while adhering to the implementation principles of; use of practical on-the-job training and mentoring; focus on key elements of RI planning and monitoring, and revisits. Routine monitoring of the approach focused on processes, outputs and short-term changesresulting from START consultants’work. From July 2013 through December 2014 three START teams of four consultants per team, worked 5.5 months each across 50 districts (45%) and five divisions of Kampala in Uganda. They conducted on-the-job training in 444 selected under-performing health facilities, with a median of two visits to each (range 1 – 7). More than half of these visits were conducted in collaboration with the district immunization officer, providing the opportunity for practice and mentorship for district immunization officer to conduct on-the-job training for health facilities. Changes in staff motivation, awareness of challenges, and completion of planning and monitoring tools were observed at both district and health facility levels. However, potential for sustainability of these, and newly introduced processes, were felt to be limited by numerous contextual factors, including external accountability, availability of resources, and individual staff motivation. Despite such limitations, mentoring and on-the-job training are promising alternatives to traditional classroom training for improving immunization program staff performance.

    Time:

    Title: Molecular Identification of Human Papillomavirus (HPV) in Cervical Smears and Parafin Embedded Tissue Samples from Women with Normal Epithelia, Low and High Grade Cervical Lesions and Cervical Cancer from the Bogota-Colombia

    Patricia Arce
    Doctor, Secretaria de Salud de Bogota-Colombia, South America

    Biography
    χ

    Biography

    Patricia Arce
    Doctor, Secretaria de Salud de Bogota-Colombia, South America



    Abstract
    χ

    Abstract

    Patricia Arce
    Doctor, Secretaria de Salud de Bogota-Colombia, South America

    Molecular Identification of Human Papillomavirus (HPV) in Cervical Smears and Parafin Embedded Tissue Samples from Women with Normal Epithelia, Low and High Grade Cervical Lesions and Cervical Cancer from the Bogota-Colombia Hernan Vargas, Ph.D1*, Patricia Arce, MPH1, Sandra Gomez MSc1, Jenny Sánchez, BSc1, Tatiana Ortiz, BSc1, Dayanne Rodriguez, MSc1, Esperanza Teuzaba, MD-Path2, Sonia Salamanca, MD3, Carlos Golijow, Ph.D4 1Secretaria de Salud de Bogotá-Colombia, South América 2 Patholab-LTDA. Clínica de la Mujer, Bogotá-Colombia, South América 3Ginesalud IPS. Bogotá-Colombia, South América 4Instituto de Genética Veterinaria Fernando Dulup. Universidad Nacional de la Plata (Argentina), South América Summary: Introduction World wide cervical Cancer is the third type of cancer affecting women. Several studies showed that in cervical cancer hpv prevalence iscloseto 90%, demonstrating that hpv infection is necessary but insufficientfor cervical cancer development. So, the development of molecular approaches to detect viral dna from oncogenic hpv types showed the benefit of diagnosingthem in high grade lesions (cin-2 and cin-3), considered cervical cancer precursors. Since these molecular techniques are sensitive, reliable and consistent, they were evaluated as tools in secondary prevention strategies and also as approaches to studing the prevalence and incidence of the viral genotypes present in sexually active women. Objetive To establish the prevalence of HPV infection in paraffin embedded samples and cervical smears obtained from women with normal cervical mucosa, high and low grade cervical lesions and cervical cancer. Methods All women belong to the public net for cervical cancer promotion and prevention program, bogotá and collected between 2015 and 2016.paraffin embedded samples were processed according totomassino et al. and also using the QIAamp DNA FFPE Tissue Kit. conventional PCR was used to amplify a región of the l1 gene from HPV using the my09/my011 primers. HPV positive samples were then genotyped using the linear array kit from roche. Results A total of 3528 samples, includingbiopsies and cervical smearswereanalyzed. HPV prevalence was 34.3% (1211/3528). HPV 16 was the most prevalent genotype found in the analyzed population, reaching 42.4% of the samples, followed by HPV 58 (10.6%), HPV 52 (8.9%) and HPV 31 (6.8%). Single infections were detected in 54.8%, (663/1211). Thehighest HPV prevalence was observed in those women among 14 and 25 years old (22.5%; n=272). The highest HPV positivity was observed in those samples classified as CIN II and CIN III (58.9%; n=274). CIn II/CIN III samples also showed the highest proportion of oncogenic HPV types. Conclusions This is the first retrospective analysis of the HPV genotypes in the sexually active women population from Bogotá, Colombia. This information could contribute to the implementation of integrated actions in order to strengthen the Cervical Cancer Detection and Control Program from the Capital City. Key Words Cervical Cancer, Cervical Intra epitelial lesions, HPV, Molecular detection

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